Kinematic marker set 2017-04-17T15:23:49+00:00

Kinematic marker set

Chimpanzee research subject with kinematic skin markers walking over force plates during kinematics/kinetics recording session. Photograph by Brigitte Demes, nd

Primate research subjects do not tolerate ball-shaped markers or marker clusters attached to them via glue or straps. Instead we used skin markers painted on with non-toxic paint.  Trial and error determined that markers consisting of white circles surrounded by a dark outline to enhance contrast on the animals’ light skin yield the best results for automatic recognition (auto-digitizing) of markers in ProAnalyst.  Animals are lightly anesthetized with isoflurane gas delivered through a mask and target areas are shaved prior to application of skin markers.  The markers included clusters of three non-colinear markers (triads) used to define anatomical segments, and additional individual markers placed on skin overlying bony landmarks associated with joint axes of rotation and / or proximal and distal segment boundaries.  To maximize visibility of the flat painted-on markers so as to minimize manual digitizing, our marker sets are designed to target left side motion only.  Several marker sets were used, but the most commonly used set for the recording of whole body kinematics will be described here.  A list of all markers used can be found in the trials that have been processed for coordinate data extraction.

Anatomical and segment marker clusters used for determining chimpanzee (A) and human (B) kinematics, from O’Neill et al., 2015, Fig. 3

Landmards for marker placement used in whole body kinematics

Pelvis
Sacrum
PSIS
ASIS
Ischial Tuberosity

Thigh
Greater Trochanter of Femur
Lateral Epicondyle of Femur
Medial Epicondyle of Femur

Leg
Head of Fibula
Head of Tibia
Lateral Malleolus of Fibula
Medial Malleolus of Tibia

Foot
Lateral Calcaneus
Medial Calcaneus
Head of Metatarsal V
Head of Metatarsal II
Distal Phalanx I
Distal Phalanx II
Distal Phalanx III

Lumbar Trunk
Spinous Process of first thoracic Vertebra
Spinous Process last thoracic Vertebra
Rib IV on ventral side

Thoracic Trunk
Spinous Process of first lumbar Vertebra
L2/L3 intervertebral Disc left
L2/L3 intervertebral Disc right

Arm
Acromion
Lateral Epicondyle of Humerus
Medial Epicondyle of Humerus

Forearm
Ulnar Styloid
Radial Styloid

Hand
Dorsum of Wrist
Metacarpal Head II
Metacarpal Head V
Metacarpal Base III
Proximal Phalanx Base II
Proximal Phalanx Base V
Distal Phalanx III

Additional Triads
Triad Thigh
Triad Leg
Triad Arm
Triad Forearm

Since most markers are on the left side of the body, they are seen by cameras when animals walked from right to left across the runway. The left side medial markers in the list as well as a set of markers on the right side are not registered in the locomotor recordings. They were located (in many experiments) with the still anesthetized animal placed in the calibrated space and a wand with several ball markers at known distances to its tip pointing to them while their left side counterparts are also visible. The right side marker positions are listed in the digitizing module of Proanalyst when applicable. Additional marker positions on the head and foot can also be found in processed ProAnalyst trials and in Thompson (head, 2016) and Holowka (foot, 2015).